Bacteria:Article Title: Compositions and methods for making antibody conjugates
Article Snippet: .. Host cells include, mammalian cells (e.g., COS-7, CV1, BHK, CHO, HeLa, LTK, NIH 3T3, 293, PAE, human, human fibroblast, human primary tumor cells, testes cells), insect cells, such as Sf9 (S. frugipeda) and Drosophila, bacteria, such as E. coli, Streptococcus, bacillus, yeast, such as S. cerevisiae (e.g., cdc mutants, cdc25, cell cycle and division mutants, such as ATCC Nos. ..
Article Title: Method for the site-specific covalent cross-linking of antibodies to surfaces
Article Snippet: .. Host cells include, mammalian cells (e.g., COS-7, CV1, BHK, CHO, HeLa, LTK, NIH 3T3, 293, PAE, human, human fibroblast, human primary tumor cells, testes cells), insect cells, such as Sf9 (S. frugipeda) and Drosophila, bacteria, such as E. coli, Streptococcus, bacillus, yeast, such as S. cerevisiae (e.g., cdc mutants, cdc25, cell cycle and division mutants, such as ATCC Nos. ..
Article Title: Proximity-based sortase-mediated protein purification and ligation
Article Snippet: .. Host cells include, mammalian cells (e.g., COS-7, CVI, BHK, CHO, HeLa, LTK, NIH 3T3, 293, PAE, human, human fibroblast, human primary tumor cells, testes cells), insect cells, such as Sf9 (S. frugipeda) and Drosophila, bacteria, such as E. coli, Streptococcus, bacillus, yeast, such as S. cerevisiae (e.g., cdc mutants, cdc25, cell cycle and division mutants, such as ATCC Nos. ..
Neutralization:Article Title: Structural basis of human cytomegalovirus neutralization by gB AD-5-specific potent antibodies.
Article Snippet: For recombinant protein expression, mammalian cells (Expi293F and HEK293S GnTI− Cells, Thermo Fisher) were cultured in suspension in GIBCO FreeStyle 293 Expression Medium (Thermo Fisher) for 5–7 days at 37◦C, with 70% humidity and 8% CO2 and rotating at 130 rpm/min. .. For neutralization assays, mammalian cells (ARPE-19 and MRC-5 cells, ATCC) were cultured in GIBCO DMEM .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA gBECD gene: (GenBank: FJ616285.1) GenScript N/A C.gBECD gene GenScript N/A AD-5 gene: (GenBank: FJ616285.1) GenScript N/A Full-length gB gene: (GenBank: FJ616285.1) GenScript N/A pcDNA3.1(+) vector Thermo Fisher Scientific Cat# V79020 Software and algorithms FlowJo BD biosciences https://www.flowjo.com/ IMGT/V QUEST IMGT https://www.imgt.org/IMGT_vquest/ PRISM Graphpad GraphPad software, LLC https://www.graphpad.com/ MotionCor2 Zheng et al.69 https://msg.ucsf.edu/software Gctf Zhang et al.70 https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/ PHENIX Liebschner et al.71 https://www.phenix-online.org/ UCSF Chimera Pettersen et al.72 https://www.cgl.ucsf.edu/chimera/ Coot Emsley et al.73 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Pymol Schrodinger, 2015 https://pymol.org/2/ RELION 3.1.1 Scheres et al.74 https://www3.mrc-lmb.cam.ac.uk/ relion/index.php/Main_Page NUIFI software Waters N/A Sequence LOGO Map GENE DE NOVO https://www.omicshare.com/ tools/Home/Soft/seqlogo Deposited data Cryo-EM structure of HCMV glycoprotein B in complex with 1B03 Fab This paper PDB: 7YRN HCMV glycoprotein B+1B03 Fab composite EM map This paper EMDB ID: 34063 Others Ficoll-Paque PLUS GE Healthcare Cat# 17-1440-02 Superdex200 column GE Healthcare Cat# 28-9909 4-12% Bis-Tris SDS-PAGE gels Invitrogen Cat# NP0321BOX Agarose Baygene Cat# BY-R0100 iBlot Gel Transfer Stacks PVDF Thermo Fisher Scientific Cat# IB24001 Ni-NTA Agarose GE Healthcare Cat# 17524801 Protein A Resin GenScript Cat# L00210 KappaSelect GE Healthcare Cat# 17-5458-01 CM5 sensor chip GE Healthcare Cat# BR100012 C1 sensor chip GE Healthcare Cat# BR100540 20 Cell Reports 44, 115646, May 27, 2025 Medium (Thermo Fisher) at 37◦C/5% CO2 and passaged every 2 or 4 days.
Cell Culture:Article Title: Structural basis of human cytomegalovirus neutralization by gB AD-5-specific potent antibodies.
Article Snippet: For recombinant protein expression, mammalian cells (Expi293F and HEK293S GnTI− Cells, Thermo Fisher) were cultured in suspension in GIBCO FreeStyle 293 Expression Medium (Thermo Fisher) for 5–7 days at 37◦C, with 70% humidity and 8% CO2 and rotating at 130 rpm/min. .. For neutralization assays, mammalian cells (ARPE-19 and MRC-5 cells, ATCC) were cultured in GIBCO DMEM .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA gBECD gene: (GenBank: FJ616285.1) GenScript N/A C.gBECD gene GenScript N/A AD-5 gene: (GenBank: FJ616285.1) GenScript N/A Full-length gB gene: (GenBank: FJ616285.1) GenScript N/A pcDNA3.1(+) vector Thermo Fisher Scientific Cat# V79020 Software and algorithms FlowJo BD biosciences https://www.flowjo.com/ IMGT/V QUEST IMGT https://www.imgt.org/IMGT_vquest/ PRISM Graphpad GraphPad software, LLC https://www.graphpad.com/ MotionCor2 Zheng et al.69 https://msg.ucsf.edu/software Gctf Zhang et al.70 https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/ PHENIX Liebschner et al.71 https://www.phenix-online.org/ UCSF Chimera Pettersen et al.72 https://www.cgl.ucsf.edu/chimera/ Coot Emsley et al.73 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Pymol Schrodinger, 2015 https://pymol.org/2/ RELION 3.1.1 Scheres et al.74 https://www3.mrc-lmb.cam.ac.uk/ relion/index.php/Main_Page NUIFI software Waters N/A Sequence LOGO Map GENE DE NOVO https://www.omicshare.com/ tools/Home/Soft/seqlogo Deposited data Cryo-EM structure of HCMV glycoprotein B in complex with 1B03 Fab This paper PDB: 7YRN HCMV glycoprotein B+1B03 Fab composite EM map This paper EMDB ID: 34063 Others Ficoll-Paque PLUS GE Healthcare Cat# 17-1440-02 Superdex200 column GE Healthcare Cat# 28-9909 4-12% Bis-Tris SDS-PAGE gels Invitrogen Cat# NP0321BOX Agarose Baygene Cat# BY-R0100 iBlot Gel Transfer Stacks PVDF Thermo Fisher Scientific Cat# IB24001 Ni-NTA Agarose GE Healthcare Cat# 17524801 Protein A Resin GenScript Cat# L00210 KappaSelect GE Healthcare Cat# 17-5458-01 CM5 sensor chip GE Healthcare Cat# BR100012 C1 sensor chip GE Healthcare Cat# BR100540 20 Cell Reports 44, 115646, May 27, 2025 Medium (Thermo Fisher) at 37◦C/5% CO2 and passaged every 2 or 4 days.
Article Title: Compounds and methods for the treatment of parasitic diseases
Article Snippet: .. Mammalian cells (HepG2, A549, and HEK293) were obtained from the ATCC and cultured routinely in DMEM with 10% FBS and 1% (v/v) antibiotic-antimycotic. ..
Article Title: Antagonizing the SAMD9 pathway is key to myxoma virus host shut-off and immune evasion
Article Snippet: .. Mammalian cells used in this study include BSC-40 (ATCC CRL-2761) (in Dulbecco minimal essential medium, DMEM, Lonza), HeLa (ATCC CCL-2 cultured in DMEM, Lonza), HEK293T (ATCC CRL-3216, DMEM), THP1 (ATCC TIB-202), and THP1-dual Lucia (Invivogen) or THP1-Lucia (kindly provided by F. Zhu Florida State University) (in RPMI1640, Lonza). .. Primary normal human CD14 + monocytes (Lonza, Walkersville, MD) (RPMI1640) and primary normal dermal fibroblasts NHDF-neo (Lonza, Walkersville, MD) (5% FBS-DMEM) were also used in this study.
Virus:Article Title: Method for inactivating zika virus and for determining the completeness of inactivation
Article Snippet: .. Any suitable cells known in the art for producing Zika virus may be used, including, for example, insect cells (e.g., mosquito cells such as CCL-125 cells, Aag-2 cells, RML-12 cells, C6/36 cells, C7-10 cells, AP-61 cells, A.t. GRIP-1 cells, A.t. GRIP-2 cells, A.t. GRIP-3 cells, UM-AVE1 cells, Mos.55 cells, Sua1B cells, 4a-3B cells, Mos.42 cells, MSQ43 cells, LSB-AA695BB cells, NIID-CTR cells, TRA-171, cells, and additional cells or cell lines from mosquito species such as Aedes aegypti, Aedes albopictus, Aedes pseudoscutellaris, Aedes triseriatus, Aedes vexans, Anopheles gambiae, Anopheles stephensi, Anopheles albimus, Culex quinquefasciatus, Culex theileri, Culex tritaeniorhynchus, Culex bitaeniorhynchus, and/or Toxorhynchites amboinensis), and mammalian cells (e.g., VERO cells (from monkey kidneys), LLC-MK2 cells (from monkey kidneys), MDBK cells, MDCK cells, ATCC CCL34 MDCK (NBL2) cells, MDCK 33016 (deposit number DSM ACC 2219 as described in WO97/37001) cells, BHK21-F cells, HKCC cells, or Chinese hamster ovary cells (CHO cells). ..
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